The Lattice-Boltzmann Modeling of Microflows in a Cell Culture Microdevice for High-Throughput Drug Screening

The aim of our research was to develop a numerical model microflows occurring in the culture chambers (CC) microfluidic device construction for high-throughput drug screening. incompressible fluid flow is based on lattice-Boltzmann equation, with an external body force term approximated by He-Shan-Doolen scheme and Bhatnagar-Gross-Krook approximation collision operator. accuracy validated algebraic solution Navier–Stokes equation (NSE) fully developed duct flow, as well experimentally. mean velocity prediction error middle-length cross-section CC 1.0%, comparing NSE solution. volumetric rate 6.1%, experimental results. analysis hydrodynamics showed that discrepancies from plug-flow-like profile are observed close inlets only, do not influence cell cultures working area CC. Within its workspace area, biochip provides stable homogeneous laminar conditions, which make procedures gradient generation, seeding, cell-staining repeatable uniform across CC, weakly dependent perturbations.